UniProtKB/Swiss-Prot PTM Description

Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Plays a direct role in facilitating neuronal migration

The single chain, almost fully active enzyme, can be further processed into a two-chain fully active form by a cleavage after Arg-310 catalyzed by plasmin, tissue kallikrein or factor Xa Differential cell-specific N-linked glycosylation gives rise to two glycoforms, type I (glycosylated at Asn-219) and type II (not glycosylated at Asn-219). The single chain type I glycoform is less readily converted into the two-chain form by plasmin, and the two-chain type I glycoform has a lower activity than the two-chain type II glycoform in the presence of fibrin N-glycosylation of Asn-152; the bound oligomannosidic glycan is involved in the interaction with the mannose receptor Characterization of O-linked glycan was studied in Bowes melanoma cell line

Glycosylation Sites

Position Structures Description Evidence
ASN-152, ASN-219, ASN-483 Associated Structures: 20 global GlycoSuite
Position Structures Description Evidence
ASN-219 OF P00750, ASN-322 OF P00749 Associated Structures: 16 global GlycoSuite
Position Structures Description Evidence
THR-96, ASN-152, ASN-219, ASN-483 Associated Structures: 15 global GlycoSuite
Site-Specific Information

A number of glycan structures have been assigned to specific glycosylation sites

Position Structures Description Evidence
ASN-322 OF P00749 Associated Structures: 13 Site specific GlycoSuite
ASN-219 AND ASN-483 Associated Structures: 6 Site specific GlycoSuite
THR-96 Associated Structures: 1 Site specific GlycoSuite
ASN-322 OF P00749 Associated Structures: 0 Site specific GlycoSuite
ASN-152 Associated Structures: 15 Site specific GlycoSuite
ASN-219 Associated Structures: 26 Site specific GlycoSuite
ASN-483 Associated Structures: 26 Site specific GlycoSuite

Notes

Accompanying information

  1. ASN-152, ASN-219{partial} AND ASN-483 [PFEIFFER ET AL. (1989) EUR. J. BIOCHEM. 186:273-286
  2. THR-96 [HARRIS ET AL. (1991) BIOCHEMISTRY 30:2311-2314
  3. ASN-152, ASN-219 AND ASN-483 [POHL ET AL. (1987) EUR. J. BIOCHEM. 170:69-75
  4. ASN-152 in UniProt (Asn117 in publication)
  5. ASN-483 in UniProt (Asn448 in publication)
  6. ASN-219 in UniProt (Asn184 in publication)

Structure Format

CFG/Essentials Text Oxford

Sequence

MDAMKRGLCCVLLLCGAVFVSPSQEIHARFRRGARSYQVICRDEKTQMIYQQHQSWLRPVLRSNRVEYCWCNSGRAQCHSVPVKSCSEPRCFNGGTCQQALYFSDFVCQCPEGFAGKCCEIDTRATCYEDQGISYRGTWSTAESGAECTNWNSSALAQKPYSGRRPDAIRLGLGNHNYCRNPDRDSKPWCYVFKAGKYSSEFCSTPACSEGNSDCYFGNGSAYRGTHSLTESGASCLPWNSMILIGKVYTAQNPSAQALGLGKHNYCRNPDGDAKPWCHVLKNRRLTWEYCDVPSCSTCGLRQYSQPQFRIKGGLFADIASHPWQAAIFAKHRRSPGERFLCGGILISSCWILSAAHCFQERFPPHHLTVILGRTYRVVPGEEEQKFEVEKYIVHKEFDDDTYDNDIALLQLKSDSSRCAQESSVVRTVCLPPADLQLPDWTECELSGYGKHEALSPFYSERLKEAHVRLYPSSRCTSQHLLNRTVTDNMLCAGDTRSGGPQANLHDACQGDSGGPLVCLNDGRMTLVGIISWGLGCGQKDVPGVYTKVTNYLDWIRDNMRP

References showing top 5 6

  1. Carbohydrate structures of a human tissue plasminogen activator variant expressed in recombinant Chinese hamster ovary cells.

    Nimtz M, Noll G, Pques E, Conradt H

    PubMed: 2226797 Year: 1990

  2. Cell-type-specific and site-specific N-glycosylation of type I and type II human tissue plasminogen activator

    Parekh RB, Dwek RA, Thomas JR, Opdenakker G, Rademacher TW, Wittwer AJ, Howard SC, Nelson R, Siegel NR, Jennings MG, Harakas NK, Feder J

    PubMed: 2514791 Year: 1989

  3. A novel sialylated N-acetylgalactosamine-containing oligosaccharide is the major complex-type structure present in Bowes melanoma tissue plasminogen activator.

    Chan A, Morris H, Panico M, Etienne A, Rogers M, Gaffney P, Creighton-Kempsford L, Dell A

    PubMed: 1823160 Year: 1991

  4. The glycosylation of Bowes melanoma tissue plasminogen activator: lectin mapping, reaction with anti-L2/HNK-1 antibodies and the presence of sulphated/glucuronic acid containing glycans.

    Jaques A, Opdenakker G, Rademacher T, Dwek R, Zamze S

    PubMed: 8687384 Year: 1996

  5. Carbohydrate structure of recombinant human uterine tissue plasminogen activator expressed in mouse epithelial cells.

    Pfeiffer G, Schmidt M, Strube K, Geyer R

    PubMed: 2513186 Year: 1989

  6. See more references
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