Man9


See UniCarb-DB MS entry UniCarbKB Search
Gas: Nitrogen
Negative
M+H M+Cl M+H2PO4
428.17 436.98 435.00
- 433.60 437.60
- 436.60 435.50
419.90 - -
436.10 - -
416.70 - -
432.70 - -
Positive
M+H M+Na M+K
436.42 434.71 437.16
443.60 445.00 447.70
Gas: Helium
Negative
M+H M+Cl M+H2PO4
335.67 346.61 341.42
Positive
M+H M+Na M+K
341.69 342.74 344.45

Man8(D1D3)


Gas: Nitrogen
Negative
M+H M+Cl M+H2PO4
408.82 412.19 416.68
406.80 - -
- 413.00 415.70
- 413.10 413.50
- 415.40 415.30
406.20 - -
408.20 - -
Positive
M+H M+Na M+K
412.80 414.64 414.72
421.40 422.80 423.20
Gas: Helium
Negative
M+H M+Cl M+H2PO4
322.98 327.88 326.05
Positive
M+H M+Na M+K
312.60 317.77 316.27

Man7(D1D1)


Gas: Nitrogen
Negative
M+H M+Cl M+H2PO4
385.29 386.03 389.12
379.90 - -
- 378.10 385.40
- 382.10 387.80
- 382.30 387.70
376.00 - -
380.80 - -
Positive
M+H M+Na M+K
389.79 384.86 387.04
391.90 393.90 398.50
Gas: Helium
Negative
M+H M+Cl M+H2PO4
295.23 295.76 298.19
Positive
M+H M+Na M+K
293.18 317.77 316.27

Man3


See UniCarb-DB MS entry UniCarbKB Search
Gas: Nitrogen
Negative
M+H M+Cl M+H2PO4
268.77 279.25 289.99
270.20 - -
- 277.30 279.40
- 277.90 280.80
266.90 - -
Positive
M+H M+Na M+K
296.78 301.51 296.38
301.10 304.60 295.30
Gas: Helium
Negative
M+H M+Cl M+H2PO4
190.05 203.11 208.53
Positive
M+H M+Na M+K
211.93 213.48 205.98

Man6


See UniCarb-DB MS entry UniCarbKB Search
Gas: Nitrogen
Negative
M+H M+Cl M+H2PO4
363.90 363.01 367.72
- 355.40 362.40
- 357.60 364.80
- 358.50 365.20
- 357.90 363.90
347.60 - -
358.20 - -
348.60 - -
359.20 - -
346.00 - -
356.80 - -
348.30 - -
358.70 - -
Positive
M+H M+Na M+K
368.85 367.03 362.46
370.10 372.50 368.60
Gas: Helium
Negative
M+H M+Cl M+H2PO4
273.36 275.35 279.30
Positive
M+H M+Na M+K
273.98 276.90 279.55

Man5


See UniCarb-DB MS entry UniCarbKB Search
Gas: Nitrogen
Negative
M+H M+Cl M+H2PO4
332.96 343.05 344.78
- 331.50 337.30
- 333.40 338.70
- 334.80 339.20
- 333.10 337.50
320.50 - -
331.30 - -
321.80 - -
332.50 - -
329.50 - -
318.40 - -
319.40 - -
330.00 - -
Positive
M+H M+Na M+K
345.35 351.48 350.33
346.60 351.90 356.10
Gas: Helium
Negative
M+H M+Cl M+H2PO4
243.24 255.63 256.29
Positive
M+H M+Na M+K
250.93 263.12 256.53

Man9Glc1


See UniCarb-DB MS entry
Gas: Nitrogen
Negative
M+H M+Cl M+H2PO4
439.13 454.41 455.18
- 452.80 451.60
429.90 - -
445.10 - -
Positive
M+H M+Na M+K
452.91 452.69 453.87
458.80 461.80 464.00
Gas: Helium
Negative
M+H M+Cl M+H2PO4
350.55 367.61 367.40
Positive
M+H M+Na M+K
- 354.33 354.74

Structure Format

CFG/Essentials Text Oxford

Ion Mobility

Method Summary

Measurements of absolute CCS values were performed using a Synapt G1 HDMS quadrupole/IMS/oa-ToF instrument (Waters Co., Manchester, U.K.) modified for drift tube operation (Bush, et al., 2010). Briefly, N-linked glycans were released with hydrazine from the well-characterized glycoproteins ribonuclease B, porcine thyroglobulin, chicken ovalbumin, and bovine fetuin obtained from Sigma Chemical Co., Ltd. (Poole, Dorset, U.K.) and reacetylated. Sialic acids were removed from the thyroglobulin and fetuin samples by heating with 1% acetic acid for 1 h at 70 °C. For electrospray analysis, samples were dissolved in water:methanol (1:1, v:v) at ∼1 mg/mL. More information refer to the supplementary section Pagel K and Harvey DJ, Analytical Chemistry 2013.

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