GlycoDigest
A tool to predict exoglycosidase digestions
Using Exoglycosidases
Exoglycosidase enzyme array digestions, in combination with U/HPLC and LC-MS, can deliver semi-quantitative glycan analysis of sugars released from glycoproteins. A detailed description and example experimental protocols for using and applying exoglycosidases is published by Royle et al., Methods Mol Biol. 2006;347:125-43.
A summary of the mode of action of exoglycosidases and examples can be found here. Click the 'Information on Glycosidases' button in the 'Build Exoglycosidases' box below.
Build Exoglycosidase Array
Use the search box below to select the panel of exoglycosidase to digest the structure shown:
| Short Name | Full Name | Source | Specificity |
|---|---|---|---|
| ABS | α(2-3,6,8,9)-Sialidase | Recombinant Arthrobacter ureafaciens gene, expressed in E. coli | α(2-3,6,8,9)-specific, cleaves all non-reducing terminal branched and unbranched sialic acids |
| NAN1 | α(2-3)-Sialidase | Recombinant Streptococcus pneumoniae gene, expressed in E. coli | Releases α(2-3)-linked sialic acid |
| BKF | α(1-2,3,4,6)-Fucosidase | Bovine Kidney | iReleases non-reducing terminal α(1-6) core-linked fucose more efficiently than other α-fucose linkages. Frequently used for release of core fucose residues |
| XMF | α(1-2)-Fucosidase | Xanthomonas manihotis | Releases non-reducing terminal α(1-2)-linked fucose |
| AMF | α(1-3,4)-Fucosidase | Almond Meal | Releases non-reducing terminal α(1-3,4)-linked fucose. Does not release core linked fucose in α(1-3,6) configuration |
| BTG | β(1-3,4)-Galactosidase | Bovine testis | Releases non-reducing terminal β(1-3,4)-linked galactose residues |
| SPG | β(1-4)-Galactosidase | Streptococcus pneumoniae | β(1-4) specific galactosidase removes galactose residues from non- reducing terminal |
| CBG | α(1-3,4,6)-Galactosidase | Coffee Bean | Hydrolyses α(1-3,4,6)-linked terminal galactose residues |
| JBM | α(1-2,3,6)-Mannosidase | Jack Bean | Releases non-reducing terminal α(1-2,6)-linked mannose residues more efficiently than α(1-3) |
| GUH | β-N-Acetylhexosaminidase | Recombinant Streptococcus pneumoniae gene, expressed in E. coli | Releases all non-reducing terminal β-linked N-acetylglucosamine but not bisecting GlcNAc β(1-4)Man residues |
| JBH | β-N-Acetylhexosaminidase | Jack Bean | Specific to all non-reducing terminal β(1-2,3,4,6)-linked N- acetylglucosamine and N-acetylgalactosamine residues |
Results
: ABS - Sialidase
This work CC BY-NC-SA 4.0
Supported by ARDC-NeCTAR | NIH Glycoscience Common Fund | Institute for Glycomics |