Colorectal Cancer Cell Line
Comprehensive glycomics comparison between colon cancer cell cultures and tumours: implications for biomarker studies
Chik JH, Zhou J, Moh ES, Christopherson R, Clarke SJ, Molloy MP, Packer NH
PubMed Entry: 24840470
Each glycan structure was quantified relative to the total content by integration of the extracted ion chromatogram peak area (EIC).
For comparison of glycan abundances the area-under-the-curves (AUCs) of each glycan structure were normalised to the total AUC (for all glycan structures in a sample summed) and expressed as a percentage.
For comparison of glycan abundances the area-under-the-curves (AUCs) of each glycan structure were normalised to the total AUC (for all glycan structures in a sample summed) and expressed as a percentage.
- Each glycan structure was quantified relative to the total content by integration of the extracted ion chromatogram peak area (EIC).
- For comparison of glycan abundances the area-under-the-curves (AUCs) of each glycan structure were normalised to the total AUC (for all glycan structures in a sample summed) and expressed as a percentage.
- Glycoproteins immobilized on PVDF membranes before N-glycans released by PNGase F, isolated and reduced
- O-glycans are chemically released from the same protein spot by reductive β-elimination
- Porous graphitised carbon liquid chromatography tandem mass spectrometry
- Negative ion mode
- Agilent LCD/MSD Trap XCT Ultra or Bruker HCT Ultra
- MS scans were between m/z 100–2200